目的 优化甘草制款冬花的微波炮制工艺。方法 以单因素实验作为基础,以紫外分光光度法(UV)和高效液相法(HPLC)测定甘草制款冬花中总生物碱、款冬酮、芦丁和异槲皮苷含量,结合醇浸出物含量的总评归一值为评价指标,对闷润时间、甘草用量、微波火力和炮制时间4个因素进行响应面实验研究,优化甘草制款冬花的微波炮制工艺。结果 甘草制款冬花的响应面法得出最佳工艺条件为闷润时间4.5 h、甘草用量15%,微波火力100%,炮制时间83 s,总生物碱、款冬酮、芦丁、异槲皮苷和醇浸出物含量分别为0.002 1%、0.283 6%、0.7%、1.44%和27.6%。结论 优选得到的甘草制款冬花炮制工艺合理、稳定、可行,可为甘草制款冬花的工业化生产提供理论依据。
Abstract
OBJECTIVE To optimize the microwave processing technology of Farfarae Flos processed with Glycyrrhizae Radix et Rhizoma. METHODS On the basis of single factor test, the index was evaluated by overall normalized value of content of total alkaloid, coltsfoot ketone, rutin, isoquercitrin and alcohol extract, which were determined by UV and HPLC method, four factors (moist time, the dosage of Glycyrrhizae Radix et Rhizoma, fire and processing time) were studied by response surface methodology. The microwave processing technology of Farfarae Flos processed with Glycyrrhizae Radix et Rhizoma was optimized. RESULTS The optimal parameters of microwave processing technology were as follows: the moist time was 4.5 h, the dosage of Glycyrrhizae Radix et Rhizoma was 15%, the microwave power was 100%, the processing time was 83 s. The contents of total alkaloid, coltsfoot ketone, rutin, isoquercitrin and alcohol extract were 0.002 1%, 0.283 6%, 0.7%, 1.44% and 27.6%, respectively. CONCLUSION The optimized processing technology of Farfarae Flos processed with Glycyrrhizae Radix et Rhizoma is rational, stable and feasible, which can provide a theoretical basis for the industrial production.
关键词
款冬花 /
响应面法 /
微波炮制 /
甘草制 /
总评归一值
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Key words
Farfarae Flos /
response surface method /
microwave processing /
being processed with Glycyrrhizae Radix et Rhizoma /
overall normalized value
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脚注
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基金
陕西省中药炮制技术传承基地建设项目资助(138010015);陕西中医药管理局中药炮制学重点学科资助(132018003);陕西省中药饮片工程技术研究中心资助(303060109);国家级大学生创新创业训练计划项目资助(201910716005)
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